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Research
(Published
online : 25-05-2013)
17. Serum biochemical and histopathological
changes in liver and kidney in lambs after zinc oxide nanoparticles
administration -
H. Najafzadeh, S. M. Ghoreishi, B. Mohammadian, E. Rahimi, M. R. Afzalzadeh,
M. Kazemivarnamkhasti and H. Ganjealidarani
Vet World. 2013; 6(8): 534-537
doi:
10.5455/vetworld.2013.534-537
Abstract
Background: Zinc is an essential
co-factor for many enzymatic activities and its deficiency may produce
clinical signs of parakeratosis, loss and failure of growth of wool and hair
in sheep.
Aim: The present study was designed to evaluate the effect of
zinc oxide nano-particles (instead conventional zinc oxide) on serum
biochemical factors and histopathological changes in liver and kidney of
lambs.
Materials and Methods: One group of lamb (4 lambs each group)
received zinc oxide nano-particles suspension daily for 25 day. Whole blood
sample and serum were collected at the start and the end of study. Activities
of lactate dehydrogenase (LDH), alanine aminotransferas (ALT), aspartate
aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN),
and creatinine (CR) were measured in serum of lambs. Sections of liver and
kidney were stained with hematoxylin-eosin and examined by light microscopy.
Results: Activity of ALP (p=0.011) was significantly decreased and
creatinine level (p=0.002) was significantly increased by zinc oxide nano-particles.
Cell swelling, eosinophilic necrosis of hepatocytes, and multifocal
interstitial nephritis was observed during histopatological examination.
Conclusion: Results of study suggest that zinc oxide nano-particles may be
toxic for use in sheep in zinc deficiency status.
Keywords:
hepatotoxicity, lamb, renal toxicity, zinc oxide nano-particles
